NHDF Glycoprotein Control Extract
| Appropriate Control For: | CMV, VZV, Rubeola Glycoproteins |
| Host Cell | Normal Human Dermal Fibroblast (NHDF) also known as HFS |
| Cell Line Characterization | Confirmed negative for HIV and Hepatitis |
| Purity | Uninfected cell extract purified over lentil lectin. |
| Inactivation | Inactivated as if infected - use as control for NHDF lentil lectin purified infected extracts |
| Supplied In | 50 mM tris, 1M NaCl, 1 mM MgCl2, 0.1% Triton X-100, 0.2M Methylmannopyrannoside pH 7.5 |
| Protein Concentration | 0.1 mg/ml |
| Fill Volume | 1.03 ml (AV043-1) or 25.2 ml (AV043-25) |
| ELISA (IgG) Titer | >1:200 |
| Western Blot Titer | N/A |
| Reference and/or Additional Reading | Exp. Cell Research (1961) 25:589 |
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ELISA Results
Antigen is Virusys # CV216 CMV Glycoproteins, VV147 VZV Glycoproteins, RV203 Rubeola Glycoproteins and #AV250 NHDF Glycoprotein Control coated at various dilutions in PBS. Primary antibodies are human plasmas previously characterized for the respective serology.
This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.
