Generate Recombinant Baculovirus from Vector
Your transfer vector is allowed to insert via homologous recombination into the baculovirus genome. Virus that has experienced a double recombination is purified away from wild type and single cross-over virus. The purity of this P1 virus is (optionally) confirmed by PCR.
3 - 4 weeks
Sufficient P1 virus is generated so as to initiate amplification for a high-titer stock
Specification sheet shows a schematic diagram of the DNA and protein sequences at the cloning junctions and shows supporting agarose/EtBr gel photos.