Sf9 Uninfected Control Extract
Appropriate Control For:
Cell Line Characterization
ELISA (IgG) Titer
Western Blot Titer
Reference and/or Additional Reading
Baculovirus/Sf9 infected cell extracts
Spodoptera frugiperda (Sf9)
Negative for mycoplasma and bacteria
Uninfected cell extract
Phosphate Buffered Saline pH 7.4
1.03 ml (AG141-1) or 25.2 ml (AG141-25)
In Vitro 13: 213-217 (1977)
Antigens are # AG141 Sf9 Uninfected Cell Extract and 2 different 6-his tagged protein extracts coated at various dilutions in PBS. Primary antibody is chicken anti-6-his at 1:1,000.
See SOP-138 for procedure.
This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.