Purpose

To describe the formulation of 10X Tris-Glycine-SDS electrophoresis running buffer.

References

Polyacrylamide gel electrophoresis in SDS containing buffers using multiphasic buffer systems: Properties of the stack, valid Rf measurement and optimized procedure. Anal. Biochem. (1978), 78:459

Materials

  • RMS-110 Tris
  • RMS-123 Glycine
  • RMS-177 Sodium Dodecyl Sulfate (SDS)

Equipment

  • Stir Plate and stir bar
  • Glassware
  • pH meter and pH standards

 

Procedure

  1. Add the following to 880 ml DI water:
         - 30.3 g Tris
         - 144.1 g Glycine
         - 10 g SDS
  2. Stir to dissolve.
  3. Standardize pH meter to pH 7 and 10.
  4. Check pH of solution. It should be between 8.6 and 8.9.  DO NOT ADD ACID OR BASE TO ADJUST pH.
  5. Label with contents, lot number and one year expiration date.

Latest Additions

Your Purchases Today

The cart is empty

OpenWetWare

vipr logo 1