To describe the formulation of 10X Tris-Glycine-SDS electrophoresis running buffer.
Polyacrylamide gel electrophoresis in SDS containing buffers using multiphasic buffer systems: Properties of the stack, valid Rf measurement and optimized procedure. Anal. Biochem. (1978), 78:459
- Add the following to 880 ml DI water:
- 30.3 g Tris
- 144.1 g Glycine
- 10 g SDS
- Stir to dissolve.
- Standardize pH meter to pH 7 and 10.
- Check pH of solution. It should be between 8.6 and 8.9. DO NOT ADD ACID OR BASE TO ADJUST pH.
- Label with contents, lot number and one year expiration date.