Purpose

To describe the formulation of 10X Tris-Glycine electrophoretic transfer buffer.

References

Western Blotting of Proteins, Clinical Biotechnology (1989); 1(1):43-49

Materials

  • RMS-110 Tris
  • RMS-123 Glycine

Equipment

  • Stir Plate and stir bar
  • Glassware
  • pH meter and pH standards

Procedure

  1. Add the following to 890 ml DI water:
         - 30.3 g Tris
         - 144.1 g Glycine
  2. Stir to dissolve.
  3. Standardize pH meter to pH 7 and 10.
  4. Check pH of solution.  It should be between 8.6 and 8.9.  DO NOT ADD ACID OR BASE TO ADJUST pH.
  5. Label with contents, lot number and one year expiration date.

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