To describe the formulation of 10X Tris-Glycine electrophoretic transfer buffer.
Western Blotting of Proteins, Clinical Biotechnology (1989); 1(1):43-49
- Add the following to 890 ml DI water:
- 30.3 g Tris
- 144.1 g Glycine
- Stir to dissolve.
- Standardize pH meter to pH 7 and 10.
- Check pH of solution. It should be between 8.6 and 8.9. DO NOT ADD ACID OR BASE TO ADJUST pH.
- Label with contents, lot number and one year expiration date.