Expression Vector Construction
There are currently many different expression vectors from which to choose. Each will have its own characteristics regarding expression induction and the presence (or absence) of a particular protein purification tag. We take the vector (or gel purified band) that you supply and ligate it into an appropriate expression vector. This new vector is then used to transform an expression strain of E. coli for production or it is used to transfer your gene to baculovirus via homologous recombination.
2 - 3 weeks
1 - 5 µg vector plus glycerol stock of E. coli strain harboring your vector.
Specification sheet shows a schematic diagram of the DNA and protein sequences at the cloning junctions and shows supporting agarose/EtBr gel photos.