Virusys Corporation’s HHV-7 qPCR 20x probe mixes are reliable probes for measuring relative amounts or total copy numbers of HHV-7 by quantitative PCR. The probes consist of forward and reverse primers combined with a FAM/BHQ-labeled probe which bind within the U57 capsid region of the genome. Each dual-labeled probe mix is at 20x concentration and may be used with standard real-time PCR reagents.
For absolute copy number calculations, a tube of standards are also included containing the target sequences at 1012 copies per ml. These standards produce linear results at dilutions of 108 to 102 copies per reaction.