E. coli IMS Bead Reagents
Escherichia coli (or E. coli for short) is a common bacterium that lives in human and animal intestines, where it is present in large numbers. There are hundreds of E. coli strains and most are relatively harmless, causing illnesses such as traveler’s diarrhea only when consumed in exceedingly high numbers. A notorious exception is E. coli strain 0157:H7, an emerging pathogen that produces a powerful toxin and can cause severe illness. E. coli O157:H7 was first recognized as a cause of illness during an outbreak in 1982 traced to contaminated hamburgers. Since seen, incidents were also reported were people became sick after drinking or swimming in water contaminated by E. coli 0157:H7. Symptoms of poisoning by E. coli 0157:H7 include bloody diarrhea, kidney damage, and occasionally death.
For the latest in published research in this area, see Bushon et al - Comparison of immunomagnetic separation/adenosine triphosphate rapid method to traditional culture-based method for E. coli and enterrococci enumeration in wastewater.
The Virusys E. coli IMS Beads have been developed for the immunomagnetic separation (IMS) of E. coli from a wide range of sample matrices.
The high sensitivity and specificity of the E. coli IMS Beads results from the covalent attachment of a high affinity polyclonal antibody developed against an epitope on the cell wall of E. coli. When combined with the Virusys IMS Assay Buffer (IMS206:BFR) and IMS Wash Buffer (IMS206:Wash), the E. coli organism can be efficiently isolated and enumerated by a variety of techniques including, but not limited to immunofluorescent antibody (IFA) staining and PCR.
The Detection Set contains 1 x 1 ml Luciferin-Luciferase concentrate and 2 x 1 ml Luciferin-Luciferase Diluent.
The Lysis Set contains 3 x 1.5 ml Somatic Cell Lysis Reagent and 1 x 1 ml Bacterial Lysis Reagent.