Cat. No. HA056-100, HA056-1
Target Protein HSV gB Envelope Protein
Target Protein Also Known As gB, pUL27, gp130
Clone Designation 10B7
Species/Isotype Mouse IgG1(k)
Purity Affinity Purified, > 95% IgG by SDS-PAGE
Supplied in Phosphate Buffered Saline pH 7.4 (no azide)
Protein Concentration 1.0 mg/ml
Fill Volume 103 µl
Immunofluorescence Titer

HSV-1     1+ @ 1:25,600
HSV-2     1+ @ 1:6,400

ELISA Titer

HSV-1     0.100 @ >1:102,400
HSV-2     0.100 @ >1:102,400

Western Blot Titer

HSV-1     > 1:12,800
HSV-2     > 1:1,600

Reference / Additional Reading Virology (1989), 172:11

HA056-100    HSV gB Monoclonal Antibody - 100 µg        $275.00 each         Add to cart

HA056-1    HSV gB Monoclonal Antibody - 1 mg        $1,050.00 each         Add to cart


Immunofluorescence Results

Procedure uses Virusys # H1G028 10-well HSV-1 slides,  # H2G029 10-well HSV-2 slides, # AG002 anti-Mouse -FITC F(ab')2 w/ counterstain and # AG065 StayBright Mounting Media. See SOP-137 for details.

HSV gB IFA

 

 

 

 

 

HSV-1                           HSV-2

HSV gB IFA Data

 


Cross Reactivity Results

Determined by IFA (SOP-137) at an antibody dilution of 1:100 with Virusys # AG002 anti-Mouse-FITC F(ab')2 w/ counterstain and # AG065 StayBright Mounting Media.

Crossreactivity Data

ELISA Results

Antigen is Virusys # H1V016 HSV-1 Infected Cell Extract and # H2V017 HSV-2 Infected Cell Extract coated at a dilution of 1:100. See SOP-138 for procedure.

HSV gB ELISA Data

Western Blot Results

Antigen is Virusys # H1V016 HSV-1 Infected Cell Extract and # H2V017 HSV-2 Infected Cell Extract at 10 µg/cm. See SOP-128 for procedure.

HSV gB Western Blot

This product is intended for research and manufacturing uses only. It is not a diagnostic device. Upon receipt, store at -20°C or below. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antibody be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to two weeks is acceptable for unused portions.

 

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