HHV-8 Real Time PCR
Virusys Corporation’s HHV-8 qPCR 20x probe mix is a reliable probe for measuring relative amounts or total copy numbers of HHV-8 by quantitative PCR. The probe consists of forward and reverse primers combined with a FAM/BHQ-labeled probe specific for ORF26 (capsid triplex protein) of HHV-8. This dual-labeled probe is supplied with PCR primers at 20x concentration and may be used with standard real-time PCR reagents.
For absolute copy number calculations, a tube of standards is also included containing the target sequence at 1012 copies per ml. These standards produce linear results at dilutions of 108 to 102 copies per reaction.