EBV Negative Control Extract Lot I1857120

Catalog Number EV139-1, EV139-25
Appropriate Control for EBV
Host Cell Human B Cell
Cell Line Characterization Confirmed negative for HIV and Hepatitis B
Purity Uninfected cell extract mock enriched for viral proteins
Inactivation Inactivated as if infected - use as control for EV012 EBV Infected Cell Extracts
Supplied In 100 mM Glycine pH 9.5
Protein Concentration 0.97 mg/ml
Fill Volume 1.03 ml (EV139-1) or 25.2 ml (EV139-25)
ELISA (IgG) Titer None
Western Blot Titer 50 µg/cm
Reference and/or Additional Reading None - proprietary human B-cell line

EV139-1    EBV Negative Control Extract -1 ml        $141.26 each         Add to cart

EV139-25    EBV Negative Control Extract- 25 ml        $1,860.41 each         Add to cart

ELISA Results

Antigen is Virusys # EV012 EBV Infected Cell extract AND # EV139 EBV Negative cell extract coated at various dilutions in PBS. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-138 for procedure.

I1857120elisa

Western Blot Results

Antigen is Virusys # EV139 EBV Negative cell extract at 50 µg/cm. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-128 for procedure and see EV012 EBV Infected Cell extract for banding patterns of sera.

H1245003western

This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.