By any chance, does your #CA005 Cytomegalovirus gB mab cross react with murine CMV gB by Western? And does it detect the full length HCMV gB, as well as the cleaved product?
While this antibody works very well in western using human CMV (AD169) as antigen, murine CMV has not yet been tested.
The banding pattern in western indicates a 116 kd and 58 kd band corresponding with the full length and cleaved products. See [Click Here] for a picture of the western.