We recently purchased EV012 EBV Infected cell Extract. As the product instruction, this product shall be stored at -70 centigrade. However, our store condtion cannot meet this requirement and the purchased products are stored at -20 centigrade. After the product thawed, it is found that there are some flocs in the bottom of the container. When adding 0.05mol/l CB buffer, these flocs cannot be dissolved.
Could you please tell us what are those flocs?
Resolution:
Sonication is recommended to resolubilize antigen precipitates. Just place the probe directly into the container, cover the opening with a paper towel and sonicate at 30% power (450W unit) for approximately 30 seconds using a 50% pulse.
A small amount of precipitate is normal but extended -20 storage will allow more to form.
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