HA018-100   100 µg

   HA018-1       1 mg

 Lot Number

D0627156

April 2009

Target Protein

Target Protein Also Known As

Clone Designation

Species/Isotype

Purity

Supplied in

Protein Concentration

Fill Volume

Immunofluorescence Titer


ELISA Titer


Western Blot Titer


Reference and/or Additional Reading

HSV ICP5 Major Capsid Protein

ICP5, VP5, pUL19, MCP, p155

3B6

Mouse IgG2b (lamda)

Affinity Purified, > 95% IgG by SDS-PAGE

Phosphate Buffered Saline pH 7.4 (no azide)

1.0 mg/ml

103 µl

HSV-1          1+ @ 1:3,200
HSV-2          1+ @ 1:3,200

HSV-1          0.100 @ >1:102,400
HSV-2          0.100 @ >1:102,400

HSV-1        >1:3,200
HSV-2        >1:3,200

J. Virology (1995), 69(1):476

Immunofluorescence Results

Procedure uses Virusys # H1G028 10-well HSV-1 slides,  # H2G029 10-well HSV-2 slides, # AG002 anti-Mouse -FITC F(ab')2 w/ counterstain and # AG065 StayBright Mounting Media. See SOP-137 for details.

Cross Reactivity Results

Determined by IFA (SOP-137) at an antibody dilution of 1:100 with Virusys # AG002 anti-Mouse-FITC F(ab')2 w/ counterstain and # AG065 StayBright Mounting Media.

ELISA Results

Antigen is Virusys # H1V016 HSV-1 Infected Cell Extract and # H2V017 HSV-2 Infected Cell Extract coated at a dilution of 1:100. See SOP-138 for procedure

Western Blot Results

Antigen is Virusys # H1V016 HSV-1 Infected Cell Extract and # H2V017 HSV-2 Infected Cell Extract at 10 µg/cm. See SOP-128 for procedure.

This product is intended for research and manufacturing uses only. It is not a diagnostic device. Upon receipt, store at -20°C or below.  Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antibody be stored in use size aliquots and thawed just prior to use. 
Storage at 2 - 8°C for up to two weeks is acceptable for unused portions.