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EBV Negative Control Extract Lot K0729161 |
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EV139-1 1 ml |
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EV139-25 25 ml |
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Lot Number |
K0729161 |
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Expiration Date |
November 2010 |
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Appropriate Control for
Host Cell
Cell Line Characterization
Purity
Inactivation
Supplied in
Protein Concentration
Fill Volume
ELISA (IgG) Titer
Western Blot Titer
Reference and/or Additional Reading |
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EBV
Human B Cell
Confirmed negative for HIV and Hepatitis B
Uninfected cell extract mock enriched for viral proteins
Inactivated as if infected - use as control for EV012 EBV Infected Cell Extracts
100 mM Glycine pH 9.5
1.04 mg/ml
1.03 ml (EV139-1) or 25.2 ml (EV139-25)
None
50 µg/cm
None - proprietary human B-cell line |
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ELISA Results
Antigen is Virusys # EV012 EBV Infected Cell extract AND # EV139 EBV Negative cell extract coated at various dilutions in PBS. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-138 for procedure.
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Western Results
Antigen is Virusys # EV139 EBV Negative cell extract at 50 µg/cm. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-128 for procedure and see EV012 EBV Infected Cell extract for banding patterns of sera.
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This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions. |
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