Virus Strain

Host Cell

Cell Line Characterization

Purity

Inactivation

Supplied in


Protein Concentration

Fill Volume

ELISA (IgG) Titer

Western Blot Titer

Reference and/or Additional Reading

AD169

Normal Human Dermal Fibroblast (NHDF)

Confirmed Negative for HIV and Hepatitis B

Lectin affinity purified

Verified by tissue culture infectivity assay.

50 mM tris, 1M NaCl, 1 mM MgCl2, 0.1% Triton X-100, 0.2M Methylmannopyrannoside pH 7.5

0.42 mg/ml

1.03 ml (CV216-1) or 25.2 ml (CV216-25)

>1:6,400

10 µg/cm

J. Med. Virology (1990), 32:189

ELISA Results

Antigen is Virusys # CV216 CMV Purified Glycoproteins coated at various dilutions in PBS. Primary antibodies are human plasmas previously characterized for CMV serology. See SOP-138 for procedure.

Western Blot Results

Antigen is Virusys # CV216 CMV Purified Glycoproteins at 10 µg/cm. Primary antibodies are human plasmas previously characterized for CMV serology. See SOP-128 for procedure.

This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C.  Product degradation will result from multiple freeze/thaw cycles.  It is suggested that the antigen be stored in use size aliquots and thawed just prior to use.  Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.