Instead of using immobilized peptide or antigen to purify antibody (see Immunoaffinity Antibody Purification), we use immobilized antibody to purify antigen. The antibody can be either monoclonal or polyclonal. Usually, polyclonal antibody gives the best chance of success due to its multi-epitope specificity.
Quantity
4 load volumes on a 5 milliliter antibody column
Catalog Number
Turnaround
AG112
2 weeks
Yield
Depending on the molecular weight and concentration of the antigen and the affinity of the antibody, yields can be anywhere from several micrograms to several milligrams. This service enables scale up estimates to be made for larger preparations.
Data Provided
Certificate of analysis that documents protein concentration and purity by reducing SDS-PAGE
Related Items/Additional Information
We will need 10 milligrams antibody and up to 100 milliliters cell culture extract to perform the purification.
Price
Relevant Hyperlinks
This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
