An extract containing your histidine tagged recombinant protein is purified using an immobilized metal column. We can use your previously developed protocol, a standard "off the shelf" protocol or an optimized protocol that results from our investigation of adsorption/elution variables (see related items). Your protein is shipped to you in as pure a state as possible in minimal time.
Quantity
Extract resulting from a 100 liter fermentation
Catalog Number
Turnaround
AG077-3
2- 3 weeks
Yield
Highly variable depending on insert size, expression promoter and host used.
We use a column appropriately sized so that the majority of the available recombinant protein is captured. The column "unbound fraction" is included in the western blot data to demonstrate this efficiency.
Data Provided
A Certificate of Analysis is provided with your purified protein that provides the following data:
Buffer formulation
Volume
Protein Concentration
Digital image of your purified protein in a coomassie stained SDS-PAGE with MW markers
Digital image of your purified protein in a western blot developed with an anti-HIS monoclonal antibody (optional).
Related Items/Additional Information
AG077-1 IMAC Purification from 5 liter Fermentation AG077-2 IMAC Purification from 20 liter Fermentation
