An extract containing your histidine tagged recombinant protein is purified using an immobilized metal column. We can use your previously developed protocol, a standard "off the shelf" protocol or an optimized protocol that results from our investigation of adsorption/elution variables (see related items). Your protein is shipped to you in as pure a state as possible in minimal time.
Quantity
Extract resulting from a 1 liter fermentation
Catalog Number
Turnaround
AG077
1 - 2 weeks
Yield
Highly variable depending on insert size, expression promoter and host used.
We use a column appropriately sized so that the majority of the available recombinant protein is captured.
Data Provided
A Certificate of Analysis is provided with your purified protein that provides the following data:
Buffer formulation
Volume
Protein Concentration
Digital image of your purified protein in a coomassie stained SDS-PAGE with MW markers
Digital image of your purified protein in a western blot developed with an anti-HIS monoclonal antibody (optional).
Related Items/Additional Information
AG077-1 IMAC Purification from 5 liter Fermentation
AG077-2 IMAC Purification from 20 liter Fermentation
AG077-3 IMAC Purification from 100 liter Fermentation
