Client supplied E. coli ( glycerol stock or streaked plate) is amplified to 200 liters in LB broth and fermented to an optical density (A600) of approximately 0.6 - 0.8 prior to induction. Protein expression may be accomplished by raising the temperature or via chemical induction (1 mM IPTG) - depending on the characteristics of the host strain and vector. Other induction methodologies may be performed if desired. Cells are collected by centrifugation and supplied as a frozen paste.
Quantity
200 liters
Catalog Number
Turnaround
AG100-4
3 weeks
Yield
Approximately 5 grams cell paste per liter is typical
Data Provided
Certificate of performance specifying fermentation parameters.
Related Items/Additional Information
AG100-1 E. coli Fermentation - 5 liters
AG100-2 E. coli Fermentation - 20 liters
AG100-3 E. coli Fermentation - 100 liters
AG100-5 E. coli Fermentation - 400 liters
