Previously titrated baculovirus is used to infect Sf-9 insect cells. The infection is allowed to progress for 3 - 4 days or until maximal cytopathic effect (CPE) is observed. Cells will be collected and extracted to your choice of extraction buffer. If the protein is secreted, the tissue culture supernatant is clarified to 0.45 µm.
Quantity
14 liters of clarified fluids or a cell pellet resulting from 14 liters
Please note that you must supply at least 1.4 x 1011 PFU for the infection. You can have this produced for you separately - see AG078 Baculovirus Expansion and Titer
Catalog Number
Turnaround
AG079-3
2 - 3 weeks
Yield
Baculovirus recombinant protein expression has been documented as high as 1 gram per liter. In practice, however, yields of 1 - 5 milligrams per liter is common.
Data Provided
A report will be provided identifying the clone designation, the host cell used and the final protein concentration of the product.
Related Items/Additional Information
AG079-1 Baculovirus Protein Expression - 1 liter
AG079-2 Baculovirus Protein Expression - 5 liter
AG079-4 Baculovirus Protein Expression - 36 liter
