Investigator supplied herpesvirus samples are used to infect monolayers of appropriate susceptible cell line at 90% confluency. Samples are tested in duplicate using a six point dilution series (normally 10^-2, 10^-4, 10^-6, 10^-8 , 10^-10 and 10^-12 – other dilutions may be specified if desired). Monolayer is examined and reported at 3, 6, 9 and 12 days based on cytopathic effect.

Quantity

10 to 19 samples

Catalog Number

Turnaround

AG047-3

3 - 4 weeks after receipt of test articles

Yield

N/A

Data Provided

Cytopathic effect scoring (+ or -) on each dilution in duplicate is reported. Data is used to calculate a tissue culture infectious dose (TCID) value representative of the amount of viable, infectious virus present in the original sample.

Related Items/Additional Information

AG047-1     Herpesvirus Infectivity Assay - 1 - 4 Samples
AG047-2     Herpesvirus Infectivity Assay - 5 - 9 Samples

This assay can be used to test inactivation methodologies or test anti-viral compounds when they are included in the virus diluent.

Price

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