Client supplied ascites or 0.45 µm clarified tissue culture supernatant is purified by various standard chromatographic methodologies. ELISA is used to document the depletion of antibody from all column effluents. The final purified antibody is assayed for protein concentration and examined for purity by coomassie stained SDS-PAGE. Greater than 90% purity is typical.

Quantity

Up to 10 ml ascites or 200 ml tissue culture supernatant

Catalog Number

Turnaround

AG095-1

1 week

Yield

Process yield is affected by a number of factors, including antibody species, isotype and secretion characteristics of the hybridoma being used for the antibody production. We document full recovery of the antibody that is supplied but cannot make specific guarantees regarding the absolute mass of protein to be recovered. Somewhat typical values may range from 1 – 3 milligrams per milliliter of ascites and 20 – 50 micrograms per milliliter of tissue culture supernatant.`

Data Provided

Protein concentration and purity is documented in a complete Certificate of Analysis that is supplied with your final product.

Related Items/Additional Information

AG095-2     Monoclonal IgA Antibody Purification - 50 ml ascites/1000 ml TC

See AG094 Monoclonal Antibody Production in Cell Culture

Price

Relevant Hyperlinks

Sample Spec Sheet (PDF-147 KB)