Client supplied protein is covalently linked to Alkaline Phosphatase (calf intestine). The procedure is optimized using a separate optimization phase where the time of conjugation and amount of enzyme used is adjusted to suit your protein. Unconjugated protein and free enzyme is removed from your conjugate to yield a pure product.

Quantity

11 - 100 milligrams

Catalog Number

Turnaround

AG090-2

2 weeks

Yield

Process yield is affected by a number of factors, including free amine content of your protein.  To adjust for these variables, an optimization is performed using a portion (0.25 mgs) of your protein prior to conjugating the entire amount supplied.  In this way, we can adjust the reaction conditions to suit your protein and give you the best results possible.

Data Provided

Protein concentration is documented (as determined using BGG or BSA standard, as appropriate, in a modified dye binding assay). Proper conjugation and purity are documented in an image of a coomassie stained SDS-PAGE gel that is supplied with your final product.

Related Items/Additional Information

AG090-1     Alkaline Phosphatase Protein Conjugation - up to 10 milligrams
AG090-3     Alkaline Phosphatase Protein Conjugation - 101 - 250 milligrams

Client needs to provide protein to be conjugated.

Price

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