Client hybridoma is cultured in RPMI-1640 supplemented with 5-10% fetal calf serum. Cell viability and antibody production are monitored as needed. When culture is scaled to the appropriate volume, the culture is allowed to plateau. When the cell viability drops to 50 - 60%, the culture is harvested and clarified through 0.45 µm filters. The clarified cell culture supernatent can be shipped back to you or it can be used as process material for further purification (see related items).

Quantity

5 liter

Catalog Number

Turnaround

AG094-2

2 - 3 weeks

Yield

Process yield is affected by a number of factors, including antibody species, isotype and secretion characteristics of the hybridoma being used for the antibody production. Somewhat typical values may range from 1 – 3 milligrams per milliliter of ascites and 20 – 50 micrograms per milliliter of tissue culture supernatant.

Data Provided

Cell culture log data and antibody concentration as determined by ELISA or protein-G loading.

Related Items/Additional Information

AG094-1     Monoclonal Antibody Production in Cell Culture - 1 liter
AG094-3     Monoclonal Antibody Production in Cell Culture - 40 liter
AG094-4     Monoclonal Antibody Production in Cell Culture - 200 liter

Investigator needs to supply frozen amp of hybridoma to be grown along with any known peculiarities of the cell line. MAP and mycoplasma testing is desirable but not required.

See AG050 IgG Purification
See AG059 IgM Purification
See AG095 IgA Purification

Price

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